Ku We, Research Computing, NUS Information Technology, on 17 April 2023
AlphaFold2 has enabled structural modelling of proteins with accuracy comparable to experimental structures. A variety of modifications of AlphaFold2 have been developed to facilitate specific applications such as ColabFold (Mirdita et al., 2022), which accelerates the program and exposes useful parameters. AlphaFold-Multimer can also be applied to screen large datasets of proteins for new protein-protein interactions (PPIs) (Humphreys et al., 2021; Bryant, Pozzati, and Elofsson, 2022) and to model combinations and their fragments when modelling complexes (Mosalaganti et al., 2022).
Alphapulldown was developed as a Python package that streamlines PPIs screens and high-throughput modelling of higher-order oligomers using AlphaFold-Multimer.